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Annual Report
(2017-2018)
English
Newsletter
April to September, Vol. 19 No. 1, 2016
RTI
Vision 2030
Vision 2030
Holidays
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  • Current
Research Achievements
1. Resource Assessment and Management
The country has significant coldwater aquatic resources in terms of upland rivers, streams, high and low altitudinal natural lakes, reservoirs that hold large population of indigenous and exotic, cultivable and non-cultivable fish species. Information on resources is obsolete and updating information is a challenge due to its kaleidoscopic topography of the sector. The fishery resources of Indian Himalayan region have been digitized.
Fishery Resources of Indian Himalayan region
Suitable sites for aquaculture can be located based on water quality, soil quality and infrastructure facilities as road connectivity, availability of seed and etc. The weightage of the criteria were analyzed through Analytical Hierarchical Process (AHP). Resource inventory is one of the significant applications of geoinformatics in fisheries management for need based planning
Fishery resource Map of Sikkim and Jammu & Kashmir
Thematic aquaculture site suitability maps of Sikkim, Himachal Pradesh, and Jammu & Kashmir
Preparation of fish biodiversity map
Primary and secondary source data on fish biodiversity were used to prepare diversity maps for different drainage in the western Himalayan region. GIS based fish biodiversity maps for major river drainages such as Indus, Sutlej, Zanskar, Beas, Chenab and Shyok in the western Himalayan region were prepared using primary and secondary data on icthyofaunal diversity. The diversity data were joined with the physical maps of the drainages. Some important biodiversity maps are being presented hereunder:
GIS Based Fish Biodiversity
map of Indus River
GIS Based Fish Biodiversity
map of Zanskar River
GIS Based Fish Biodiversity
map of Shyok River
GIS Based Fish Biodiversity
map of Sutlej River
GIS Based Fish Biodiversity
map of Beas River
GIS Based Fish Biodiversity
map of Chinab River
Ichthyofaunal diversity, habitat status and hydrobiology of the rivers Western Ramganga and Kosi are being meticulously investigated.The important ichthyofauna recorded were Tor putitora, Naziritor chelynoides, Schizothorax richardsonii, Barilius bendelisis, Garra gotyla gotyla, Glyptothorax sp. and Crossocheilus latius latius.
Field surveys conducted at snow-fed river Dirang chu, river Sangti and river Tenga of Kameng drainage of West Kameng district of Arunachal Pradesh. Identified and documented five (5) species of Schizothoracids ie. Schizothorax richardsonii, Schizothorax labiatus,Schizothorax esosinus, Schizothorax progastus and Schizopyge niger based on morphological characteristics, meristic counts and by using tools of truss morphometry.
Fish specimens collected from Western
Under the eco-biological study of selected Himalayan mountain lakes, a new species of copepod of the genus Hesperodiaptomus, having high concentration of carotenoids was discovered from lake Maheshwar kund situated in Munshiyari, Uttarakhand
Under the national network project, coldwater fish germplasm repository centres have been established at Bhimtal and Champawat, with nearly 3400 specimens of indigenous coldwater fishes collected from different upper reach tributaries of river Ganga. For stock enhancement and conservation, breeding of Garra gotyla was successfully attempted.
2. Aquaculture
Polyculture of exotic carp in polylined tanks at mid-altitudinal regions (800-2000m MSL)
Earthen ponds lined with polythene sheets (LDPE) have been found suitable for fish culture in mid-altitudinal hill areas. These tanks are also used for rainwater harvesting in uplands areas where scarcity of water becomes major bottleneck in agricultural production. The use of polylined pond/tanks are advantageous for increasing fish production as water temperature during winter remains comparatively higher and support sustenance of natural food for growing fish.
Induced breeding and seed production of Indigenous minor carp Labeo dyocheilus Labeo dero and Labeo pungusia
Labeo dyocheilus (McClelland) and Labeo dero (Hamilton, 1822) are important indigenous cold water fish species, endemic to the Himalayas and inhabiting upland streams and rivers at an elevation of 400-800M. Breeding and seed production technology for both the species has been developed by DCFR, Bhimtal. Water temperature ranging 18-220C found suitable for the spawning and egg incubation in coldwater condition. Successful breeding technique in captive condition enabled seed production for wild stock augmentation and species diversification in coldwater aquaculture.
Labeo pungusia is threatened species as per IUCN but having good potential for food fish in North-Eastern India. To conserve and rehabilitate the species, successful breeding achieved at Eco-camp, ABACA, Nameri, Assam for conservation and species diversification of aquaculture in North East hill region.
Successful larval rearing of Puntius ticto,  Naziritor chelynoides, N. hexagonolepis in controlled condition in Aquarium was achieved with zero water exchange system. Due to the ammonia low level (<0.1 ppm) and high dissolved oxygen, survival (95%) and growth was recorded 3 times better than in FRP tanks.
  • Produced 6000 fingerlings of N. hexagonolepis Barilius bendelisis and Naziritor chelynoides.
A prototype zero water exchange hatchery device was designed for rainbow trout and other aquaculture candidate coldwater fish species based on water re-circulation and bio filtration.  Device is efficient for optimal use of water, better recovery with high dissolved oxygen and low ammonia.Device is a glass aquaria having dimension of 90x45x60cm and water holding capacity of 120-150 litres equipped with bottom sand and gravel bed. Gravel bed was matured with nitrifying bacteria and continuous circulation of water was maintained by a power-head pump. Production of 1200-1500 fry from the device in 60 days can be achieved without exchange of water with better survival and uniform size, while140000 litre of water is required in flow through system
Egg incubation of Labeodyocheilus
 
First Report: Successful breeding of Chagunius chagunio and Barilius bendelisis
Chagunius chagunio, belongs to family cyprinidae commonly known as "Chaguni" is one of the economically important indigenous fish having its distribution in Brahmaputra and Ganga drainages along the Himalayan foot hills. In Kumaon Himalayan region, C. chagunio is locally called as "Chippan" has high consumer preference and fetch very good market price of about Rs. 300/kg. Captive reared brood stock of this minor carp was successfully bred under coldwater conditions and breeding protocol was developed for the seed production.
   
The hill trout cyprinid, Barilius bendelisis (Hamilton), has recently drawn the attention as one of the potential candidate species for aquaculture. It is commonly distributed in Brahmaputra and Ganga drainages along the Himalayan foot hills. As per IUCN red list (2012) this fish has been categorized as least concern but in future the major threat to this species is over exploitation, habitat destruction due to natural and human interruption. Therefore, in order to overcome this problem, breeding and propagation of B. bendelisis is a prerequisite task to replace the stock. Barilius bendelisis being a demanding ornamental as well as potential food fish has not received any research attention for breeding and seed production and fish has been successfully bred for first time.
Captive breeding of Labeo pungusia  
Labeo pungusia is threatened species as per IUCN but having good potential for food fish in North-Eastern India. To conserve and rehabilitate the species, successful breeding achieved at Eco-camp, ABACA, Nameri, Assam for conservation and species diversification of aquaculture in North East hill region.
  Mature specimen of Labeo pangusia
Pond culture of Bangana devdevi  
Bangana devdevi is potential species of Manipur, locally known as  Khabak. Hence, ICAR-DCFR with Krishi Vigyan Kendra, Thoubal, Manipur have undertaken initiative to culture this fish in farmers pond.
Rearing of fingerlings of Himalayan Golden Mahseer (Tor putitora) in floating cages
Open water bodies in hills provides opportunities for in situ seed rearing in floating cages which may be used for the stock enhancement programmes. Protocol for in situ rearing density of golden mahseer upto advanced fingerling stage have been developed with appropriate stocking density and feeding practices.
Promotion of rainbow trout (Oncorhynchus mykiss) farming in Sikkim
The Directorate has provided technical support to state for promotion of trout culture. State has achieved total production of rainbow trout around 110 tonnes from both public sector and in private sector having 220 private trout raceways in the state. Most of the production is consumed locally in households and in some high class hotels. Trout farming is a viable livelihood option for the local population with high market demand in the higher altitudinal areas. A constant technical support has been provided by the DCFR, Bhimtal for infrastructural development, brood stock maintenance and seed production, feed formulation including advisory service and hands on training to the trout growers. Healthy brood stock of Rainbow trout has been maintained at State trout farm, Uttarey and produced 1.5 lakh advanced fingerlings and 3 lakh eyed ova of Rainbow trout during this breeding season. A wooden stripping stand was designed by DCFR for easy stripping operation and to reduce the man power, physical stress on brooder and on breeder during stripping operation. The device was practically demonstrated to the farmers.
DCFR rehabilitated natural disaster affected 6 tribal farmer's families at Upper Rimbi, West Sikkim and provided financial & technical support for raceway renovation, trout seed stocking and trout feed. Seed of Rainbow trout was stocked in those renovated raceways during the month of May, 2014 and achieved good growth with best management practices. Technical advice was provided to the trout growers for maintaining sufficient water flow, appropriate design of outlet, optimum stocking density and feeding management. Individual selection of the brood stock was carried at Uttarey for the stock improvement with the target of 5 lakh eyed ova production in the next breeding season
Promotion of trout culture in Highlands of Leh & Ladakh
Leh- a tribal district has low opportunity of agriculture due to harsh climatic conditions while trout culture can be adopted as small scale enterprises which can provide round the year livelihood support to the dwellers. ICAR-Directorate of Coldwater Fisheries Research, Bhimtal has initiated trout faming programme under Tribal Sub Plan for improving socio-economic status of the tribal population during 2013-14 in collaboration with HMAARI (SKUAST-K) RRS- CAZRI (ICAR)-Leh and Department of Fisheries, Govt. of Jammu & Kashmir. Initially three (3) existed raceways were repaired and 5 new raceways were got constructed in Chushout Shamma village which is about 20 km from the Leh and located near the bank of a tributary of River Indus at 3280 meter above sea level. Presently, six (6) tribal families are covered under trout farming programme and a total of 13 trout raceways have been created. Technical know-how and inputs (trout seed and feed) provided to the farmers.
Trout seed and feed distribution
   
A View of trout raceways Creating awareness in Kisan Mela
3. Fish Health Management
Pathogenic bacteria profile of rainbow trout farms, Champawat (Uttarakhand), Dachigaon, Kokernag (Jammu & Kashmir), Kullu & Jogindernagar (Himachal Pradesh) is developed
Bacteria in group Aeromonand [Aeromonas hydrophila RTK08 (KC603616), RTMCX1 (JX390650), RTG33 (KC603615), Aeromonas allosaccharophila RTGT19 (KC603617), RTKKO1 (KC816585), Aeromonas veronii (A.  ichthiosmia) RTCE02 (KC582608), RTGBCX2 (JX390651), RTGBCX2 JX390651.1, Aeromonas sobria RTSKO3 (KC816586), Aeromonas popoffii RTGL26 (KC603614)] frequently isolated from tissue samples of rainbow trout, Oncorhynchus mykiss are considered as predominant, opportunistic pathogen in trout farms.
Fourstrains of Lactococcus garvieae (GenBank accession no: KM 604701, KM 604702, KM 604703 and KM 604704) are isolated & identified from diseased rainbow trout showing typical symptoms [unilateral exophthalmia, inflammation and swollen vent, petechial haemorrhages in viscera, focal haemorrhages and swelling in liver] of lactococcosis from Uttarakhand and Himachal Pradesh.    
Antibacterial susceptibility test of Enterococci isolated from diseased eye (corneal opacity) of rainbow trout from Jogindernagar, Himachal Pradeshshowed resistant to Nalidixic acid(Na30), Ketocomazole(Kt10), Clindamycin(Cd2), Lincomycin(L2), Cephalexin(Cp30), Polymyxin B(Pb50), Amphotericin B(AP100), Ceftazidine-Ca30/10, Cloxacillin Cx5, Metronidazole (MT5), Fluconazole (Fu10), Fluconazole (Fu25), Amphotericin B(AP20), Itraconazole(It10), Oxacillin (Ox5), Sulphamethoxypyridazine(St300), Methicillin (Met10) out of 36 tested market antibiotics. 
Green-yellow pigmented strain of Pseudomonas koreensis, TPEB02, (JX390644) is recovered from the golden mahseer eye, demonstrating opacification of the spectacle in conjugation with increased prominence of vessels.  
Pathogenecity of Pseudomonas koreensis, TPEB02, (JX390644) is established through experimental infection of 12hr cultures of P. koreensis to golden mahseer fingerlings.
Emerging fish pathogen, Chryseobacterium scophthalmum (KM822770) is isolated from golden mahseer gill in Bhimtal Lake showing clinical symptoms bacterial gill disease. Detail study of prevalence & patho-physiology of this pathogen is under investigation. 
Occurrence of corneal hypertrophy is observed in fingerlings of chocolate mahseer, Neolissochilus hexagonolepis (McClelland, 1839) from Uttarakhand, India.
Incidence of viral diseases of cold water fish species from the States of Jammu & Kashmir, Himachal Pradesh, Uttarakhand, Sikkim and Aruanchal Pradesh are being investigated under NSPAAD.
 
  • Documented aqua-drugs and medicines from Uttarakhand (45 farms), Himachal Pradesh (26 farms), Sikkim (68 farms), Tripura (62 farms), Meghalaya (65 farms) and Arunachal Pradesh (30 farms)
  • Salt, lime, KMnO4, cypermethrin-high cis (TIK-OUT), WASORICH (probiotics), Q4ALL (for broad spectrum biodegradable disease control), aqua promass and aquamin (as growth promoters), tetracycline hydrochloride, malachite green, betadine, CIFAX, bleaching powder, clinar, apiclin, vazra, domax are commonly applied in above coldwater states.
  • Under the National Surveillance Programme on Aquatic Animal Diseases, 25 rainbow trout and carp farms of Himachal Pradesh, Jammu & Kashmir and Uttarakhand were surveyed for the presence of coldwater fish diseases. The results indicated the absence of infectious pancreatic necrosis virus and viral haemorrhagic septicemia virus.
  • Under the All India Network Project on fish health, primary data concerning the use of aqua drugs, medicines, antibiotics and treatment kits were collected from nearly 300 fish farms in six hill states. Table salt, lime and potassium permanganate were the commonly used agents for fish health management in hill aquaculture practices.
  • Mx gene and Mx promoter of snow trout have been identified and characterized at the molecular level in the first phase of an approach that aims to develop a method for detecting the presence of virus signal in clinical samples of fish.
  • 10 bacterial isolates were identified and characterized based on their growth inhibiting and algicidal activities, in order to develop a bacterial bioremediation measure that can mitigate Microcystis aeruginosa blooms in aquaculture ponds and water bodies.
Figure 1a: Unilateral exophthalmia & b: Inflammation & swollen vent showing typical symptom of Lactococcosis, c: Golden mahseer (Tor putitora) eye, demonstrating opacification of the spectacle conjugation with increased prominence of vessels, d: Golden Mahseer (Tor putitora) showing symptoms of Bacterial Gill Disease (BGD) collected from subtropical Himalayan Lake Bhimtal, e:   Corneal hypertrophy observed in fingerlings of chocolate mahseer, Neolissochilus hexagonolepis (McClelland, 1839).
4 Fish Nutrition and Feed Development
Azolla based wet farm made larval & grow out feed developed for minor carp, Labeo dyocheilus (McClelland), which enabled seed production and aquaculture of this important indigenous cold water fish species in lower and mid Himalayan region.
Protocols for the successful culture of Chlorella and rotifer for feeding of mahseer larvae were developed
The protocol for preparation of microparticulate diet for golden mahseer larvae has been established and based on this protocol, a microparticulate diet "NANHE MAHSEER" for feeding of golden mahseer, Tor putitora larvae has been developed and evaluated.
Cost effective growout feed with 1.43 FCR has been developed and evaluated for culture of chocolate mahseer, Neolissocheilus hexagonolepis.
Grow out feed for the snow trout has formulated with minimum use of fish meal.
Starter rainbow trout feed is prepared with blend of animal & plant protein, which is more efficient over the commercial feed and is a cost effective feed.
Formulated a vitamin and mineral premix which sufficiently meet the requirement of rainbow trout when supplemented in their diet at a level of 0.5 and 1%, respectively.
Grow out feed for rainbow trout has formulated with minimum use of fish meal & fish oil.
Farm made trout feed using fish offal/poultry offal has been demonstrated to the farmers of Sikkim & Himachal Pradesh. This readily available material based feed is using by the farmers as emergency feed, when commercial feed is not readily available for a short period.
5. Coldwater Fish Molecular Genetics
a. Molecular Genetics
1. Development of robust RACE methodology and characterization of growth, metabolism and maturation related gene markers
Random amplification of cDNA ends (RACE) is a PCR technique used for amplification of either 5’or 3’ ends of a cDNA. It is also used to identify isoforms, variants and different forms of the same gene. Considering the constraints and limitations in using commercial kits, we standardized an in-house 5’ RACE technique based on the template switching ability of MMLV reverse transcriptase H minus and 3’ RACE based on step out PCR technique. Importantly, this in house RACE method was 30-40 times cheaper than commercially available RACE kits.
5' RACE and 3' RACE products of aromatase genes from snow trout (M: 1 kb DNA marker, Lane 1: 5' product of cyp19b, Lane 2: 3' isoforms of cyp19b, Lane 3: 3' product of cyp19a)
2. Bioprospecting of genes and allele mining for thermal stress tolerance of Schizothorax richardsonii
Members of the suppressor of cytokine signaling (SOCS) family are crucial for the control of a variety of signal transduction pathways that are involved in immunity, growth, and development of organisms. The orthologs of SOCS-1a and SOCS-3a genes in Schizothorax richardsonii were identified using specific primers designed from transcriptome database and available teleost SOCS family genes.
3. Transcriptome analysis coldwater fishes against Aeromonas hydrophila infection
Transcriptome analysis of the response of golden mahseer to Aeromonas hydrophila infection indicated that transcripts associated with the complement system, antimicrobial peptides, immune responsive acute phase proteins, and other stress-related proteins trigger the adaptive mechanism to counter bacterial infection

Transcriptome analysis of heat shock responsive transcripts in Schizothorax richardsonii revealed that out of 20,506 unigenes annotated into 35 functional groups, genes related to the regulation of metabolic processes was an integral part of thermal tolerance. In silico transcriptome analysis of the response of golden mahseer to Aeromonas hydrophila infection indicated that out of the 2,408 differentially expressed transcripts, the majority were found to be involved in the reorganization of the cellular system.
4. Development of fish viral peptide based nano system
Three peptide nano systems were designed from fish viral proteins, synthesized and purified. Further, the cargo carrying capacity of one of the cell penetrating peptides has been successfully validated in vitro using plasmid DNA containing GFP gene.
Delivery of GFP gene using RR28 peptide in fish cells (CHSE-214)
5. Mitogenomes analysis of coldwater fish genetic stock
The complete mitogenomes of brown trout and chocolate mahseer have been characterized through next generation sequencing. The genetic differences between geographically distinct chocolate mahseer and brown trout populations are being analysed using mitochondrial gene markers. Moreover, a partial genomic library was constructed and used to isolate SSR markers for genotyping the 11 chocolate mahseer and 5 brown trout populations. Similarly, 3876 SSR containing sequences were obtained from the partial genomic library of Schistura sikmaiensis. Among the 32 loci selected for genotyping the different populations, 18 loci were found to be highly polymorphic.
b. Genomic Resources
1. Mitochondrial DNA sequences:
Table: Mitochondrial DNA sequences
Species Cyt b ATPase6/8 COI COII 16 SrRNA
Tor putitora 170 161 6 10
Tor khudree 4
Tor chelynoides 4
Neolissochilus hexagonolepis 5
Schizothorax richardsonii 47 25 22 25 10
Schizothorax progastus 5 5 5 5
Schizopyge niger 3 5 5 5
Schizothorax esocinus 4 5 5 5
Barilius bendelisis 15 6
Garra gotyla 8
Acanthocobitis  botia 2
Schistura obliquofascia 5
Schistura rupecula 7
Glossogobius gutum 6
Glossogobius giuris 4
Glossogobius spp.     6    
2. Complete Mitochondrial Genome Organization of Coldwater Fish Species
The complete mitochondrial genome of important coldwater fish species under genus Schizothorax (snow trout) and Tor (mahseer) was determined for the first time. This study will provide the rationale for the management and conservation of the endemic coldwater fish species.
Table: Details of complete mitochondrial genomes of different coldwater fish species

Species

Genome Size (bp)

GenBank
Accession number

Protein coding genes

tRNA

rRNA

A + T content

G + C content

Schizothorax richardsonii

16,592

KC790369

13

22

2

55.4%

44.6%

S. labiatus

16,582 

KF739398

13

22

2

55.3%

44.7%

S. progastus

16,575

KF739399

13

22

2

55.2%

44.8%

S. esocinus

16,583 

KF600713

13

22

2

55.2%

44.8%

S. plagiostomus

16,576 

KF928796

13

22

2

55.8%

44.2%

Schizopyge niger

16,585

NC_022866

13

22

2

55.2%

44.8%

Tor putitora

16,576

KC914620

13

22

2

56.9%

43.1%

Species Characterization
Mitochondrial Genome organization of S. richardsonii Mitochondrial Genome organization of Tor putitora
3. Development of Microsatellite Markers
Microsatellite markers in important coldwater fish species were developed. The informative loci can be used for population genetics studies and long-term endeavours such as genome mapping, localization of quantitative trait loci and the implementation of marker-assisted selection.
Table: Development of microsatellite marker from genomic library

Species

No. of Markers developed

GenBank
Accession number

Schizothorax richardsonii

51
26 (ESTs SSR)

HM591233-HM591283
JK087- 411, 471, 486, 532, 553, 716, 738, 740, 763, 886, 902, 903, 992, JK088032, 043, 048, 052, 064, 073, 103, 106, 195, 201, 219, 319, 390

Schizopyge niger

12

KC339275-KC339286)

Garra gotyla

52

HQ288484-HQ288526 & JF268657-JF268665

Tor putitora

22
34 (EST SSR)

JX270775-JX270794

Schistura sikmaiensis

36

KJ545923-KJ545958

Neolissocheilus hexagonolepis

12

validation in process

4. Development of EST Markers from cDNA of Schizothorax richardsonii
The snow trout (Schizothorax richardsonii) is an economically important fish in the north-east Himalayan region. However, genomic research on this species is still in its infancy, and genomic resources are largely unavailable. The objective of this study was to generate expressed sequence tags (ESTs) from a brain complementary DNA (cDNA) library and to identify the genes. A total of 1031 ESTs were sequenced, from which 73 contigs and 411 singletons were identified. BLAST homology analysis indicated that only 9.3% of these ESTs were homologues of known genes while the remaining 90.7% appeared to be novel sequences. Based on sequence similarities, 45 putative genes were identified that encodes stress proteins, enzymes and signal transduction regulators. Our study thus, provides a collection of novel transcripts and a partial annotation of genes that are expressed in brain tissue of the snow trout species, S. richardsonii.
Table: ESTs from cDNA of Schizothorax richardsonii

Description

Number

Total cDNAs Sequenced

1031

Total analysed cDNAs

1023

Average EST size (bp after trimming)

442

Total number of Unigenes

484

Number of Contigs

73

Number of orphan sequences (Singletons)

411

Unigenes with known gene match n(%)

45 (9.3%)

Unigenes with no match, n (%)

439 (90.7%)

  Neolissocheilus hexagonolepis
Species and Population Characterization of Coldwater Fishes Using Mitochondrial and Microsatellite Markers
1. Novel species identification - Schistura obliquofascia, a new loach from Uttarakhand, India
  • Amplicon of 307 bp of Cyt b region of the mtDNA was amplified using Universal Primers (Kocher et al. 1989).
  • L14841AAAAAGCTTCCATCCAACATCTCAGCATGATGAAA
  • H15149-AAACTGCAGCCC CTCAGAATGATATTTGTCCTCA
  • Sequences of the mitochondrial Cyt b genes of the other two species of Schistura and Schizothorax richardsonii were used for phylogeny.
  • Neighbor-joining tree and genetic distance data show S. corica to be genetically distant from the S. obliquofascia and S. beavani.
2. Species characterization using mitochondrial DNA (mtDNA)
Species were characterized using mtDNA (Cytb, ATPase 6/8, Cytochrome Oxidase I, Cytochrome Oxidase II and 16 SrRNA) markers.
  • Tor (T. putitora, T. tor, T. khudree T. chelynoides). N. hexagonolopis (Fig. A).
  • Four coldwater species (G. gotyla, B. bendelisis, S. richardsonii and T.putitora) (Fig. B)
  • Genus Schizothorax (S. richardsonii, S. esocinus, S. niger, S. progastus, S. plagiostomus) (Fig. C)
2. Species characterization using mitochondrial DNA (mtDNA)
Mitochondrial gene sequences and microsatellite markers were used to study the genetic differentiation and phylogenetic relationships between species and populations of coldwater fish species from different geographic regions of India.
Table: Population characterization of important coldwater fish species

Species

Type of Molecular marker

MtDNA  genes

Microsatellite

Tor putitora

 Cyt b (1140 bp) and ATPase6/8 (842 bp)

Cross species amplification through 13 microsatellite primer.

Schizothorax richardsonii

Cyt b (1140 bp) , ATPase6/8 (842 bp), COI (847 bp) and COII (560 bp)

28 developed
Microsatellite marker.

Barilius bendelisis

Cyt b (307 bp)

Cross species amplification through 13 microsatellite primer.

3. Genetic stock assessment of farm raised rainbow trout (Oncorhynchus mykiss) in India
  • The rainbow trout (Oncorhynchus mykiss) in India is a century old introduction, supporting trout farming in most of the cold water regions.
  • Genetic stock assessment of farmed rainbow trout from different coldwater regions of India was done for the first time.
  • Samples from Western Himalayas (Kashmir), Central Himalaya (Uttarakhand) and Southern India (Munnar hill) were evaluated for allelic and gene diversity using 15 polymorphic microsatellite loci.
  • This study revealed that RTMK (Munnar, Kerala) and RTDK (Dachigam, Kashmir) formed individual clusters while RTBU, RTCU (Bairangana and Champawat, Uttarakhand) and RTPH (Patlikul, Himachal Pradesh) stocks were grouped in another cluster indicating a common origin of the culture stocks.
  • Substantial genetic differentiation was observed among stocks, low inbreeding level and absence of bottleneck.
  • The study will provide the rationale for the management of farmed rainbow trout in India.
Functional Genomics
• Physiology of cold acclimation in Schizothorax richardsonii and Barilius bendelisis
Glycerol accumulation in blood plasma and modulation in the activities of selected enzymes associated with glycerol synthesis in the liver under cold temperature were assessed in two freshwater cyprinid fishes, Schizothorax richardsonii (snow trout) and Barilius bendelisis (Indian hill trout). The decrease in temperature triggered higher accumulation of glycerol in the blood plasma of both the species. Glycerol accumulation in plasma was found to be associated with changes in enzyme in the liver. In both the species, liver exhibited higher activities of glycerol-3-phosphate dehydrogenase (GPDH) and higher concentration of dihydroxy acetone phosphate (DHAP) which gets converted into glycerol. Based on the enzyme profile studied, it was established that the freshwater cyprinids; S. richardsonii and B. bendelisis, a decrease in water temperature triggered the release of glycerol in the blood plasma and also activated metabolic response for glycerol accumulation. The higher accumulation of glycerol seems to contribute towards cold adaptation in these species.
• Expression profile of snow trout (Schizothorax richardsonii) GPDH gene in response to abiotic stress
Glycerol-3-phosphate dehydrogenase (GPDH) gene possibly plays a key role for cold acclimation process in snow trout during winter months when water temperature goes down to 4-5 0C. In S. richardsonii the qRT-PCR showed highest expression in muscle tissue followed by liver and also revealed 19 fold gene expression in liver tissue under cold (5 0C) in comparison with warm (15 0C) condition. The elevated expression levels of GPDH cDNA on cold treatment suggested that GPDH plays a role in stress related responses in S. richardsonii.
• Expression profile of snow trout (Schizothorax richardsonii) Mannose Binding Lectin gene in response to biotic stress
Teleost fishes mostly depend on their innate defense mechanisms, like LCP (Lectin Complement Pathway) to protect themselves from invading pathogens. Mannose binding lectin (MBL) is a member of the collectin family of proteins that plays an important role in the innate immune response. Its deficiency is often being associated with increased risk, severity and frequency of infections. The study was undertaken to identify and characterize MBL or its homologue in snow trout challenged with Aeromonas hydrophila (MTCC 646). Snow trout MBL homologue (Sch-GBL) was cloned and characterized for the first time in any Coldwater indigenous fish species in the high altitude Himalayas. The MBL homologue isolated in this study was found to be specific for galactose (Sch-GBL). Regulation of Sch-GBL by bacterial infection and downshift of temperature at transcription level was also studies. The present study also showed that Sch-GBL acts as an acute phase reactant after a bacterial infection (A. hydrophila).
Notice Board
Nomination for ToT program
Nominations invited for ICAR-DCFR Training (ToT) programme during 27-31 August, 2018
 
Fish farmers Day
ICAR-DCFR, Bhimtal celebrated the fish farmers Day on 10.07.2018
 
Rainbow Trout Farming
ICAR-DCFR Demonstrated Rainbow Trout Farming at Munsyari, Uttarakhand
 
Skill Development Programme
ICAR-DCFR, Bhimtal organized a skill development programme on Start up fish farming, seed production and hatchery management for hill farmers of Ziro valley, Arunachal Pradesh
 
Visit of Smt. Rekha Ayra
Visit of Smt. Rekha Ayra, Honble Minister of Women Welfare & Child Development, Animal Husbandry, Sheep & Goat Husbandry, Fodder & Meadows Development and Fisheries, Govt. of Uttarakhand at Field Centre, ICAR-DCFR, Champawat on 3.5.2018
 
Visit of Honble Minister
Visit of Honble Minister of state for Agriculture & Farmers Welfare (Government of India) at ICAR-DCFR, Bhimtal on 13.5.2018.
 
ICAR-DCFR, Bhimtal developed mahseer hatchery and brood bank at Mokokcheng, Nagaland
ICAR-DCFR, Bhimtal developed mahseer hatchery and brood bank at Mokokcheng, Nagaland.
 
Awareness Workshop
Awareness Workshop on Protecting Intellectual Property Rights on 20 March, 2018
 
Orientation Programme
ICAR-DCFR, Bhimtal Organised Start-up orientation programme on Ornamental Fish Aqua-gardening for Tribal Students.
 
Interactive Meet
ICAR-DCFR, Bhimtal and Gaumco Society organized Farmers-Officers-Scientists Interactive Meet at Ziro for Enhancing Fish Farmers Income
 
Awareness-cum-training programme
Awareness-cum-training programme on Avenues in Fisheries to Combat Climate Change in High Altitude on 12th March 2018
 
Kisan Mela
ICAR-DCFR, Bhimtal organized Kisan Mela at Experimental Fish Farm & Field Centre, Champawat (Uttarakhand) on February 24, 2018
 
International Workshop
International Workshop on Charting the Innovative and Strategic Paths for Rehabilitation, Propagation and Conservation of Mahseer
 
Press Report
Himani Aquarium at ICAR-DCFR, Bhimtal is now open for public
 
Visit of Shri R.P. Singh
Visit of Shri R.P. Singh, Hon’ble Member, Governing Body, ICAR Society, New Delhi
 
Sangoshthi
One-Day Sangoshthi on "Promotion of Mahseer Eco-tourism in Kumaon Region"
 
WORLD SOIL DAY
ICAR-DIRECTORATE OF COLDWATER FISHERIES RESEARCH, BHIMTAL Organized WORLD SOIL DAY on 5th December, 2017
 
AGRICULTURAL EDUCATION DAY
ICAR-DIRECTORATE OF COLDWATER FISHERIES RESEARCH, Bhimtal Organized AGRICULTURAL EDUCATION DAY on 3rd December, 2017
 
National Workshop
Recommendations of National Workshop on Mahseer in Recreational Fisheries and Eco-tourism in NE India
 
National Seminar
Recommendations of "Strategies, Innovations and Sustainable Management for Enhancing Coldwater Fisheries and Aquaculture"
 
Vigilance Awareness Week
Vigilance Awareness Week at ICAR-DCFR.
 
National Seminar
ICAR-DCFR, Bhimtal Organised National Seminar during 22-24 September, 2017
 
Awareness Cum Training Programme
ICAR-DCFR organized Awareness Cum Training Programme on Fish Health Management and ornamental fishes on 20.8.2017 under New India Mission: Sankalp se Siddhi
 
Abstract Submission
Last date of submission of abstract extended till 20th August 2017
 
Awareness Cum Training Programme
ICAR-DCFR organized Awareness Cum Training Programme on Fish Health Management in Coldwater Aquaculture on 29.7.2017
 
Training Programme
ICAR-DCFR along with SAMETI-Jammu organized three days training programme on Fish Breeding Techniques on 24.7.2017
 
Invitation for the National Seminar
Invitation for the National Seminar during 22-24 September, 2017 at DCFR, Bhimtal. Looking forward to welcoming you.
 
Fish Farmers Day
ICAR-DCFR celebrated Fish Farmers Day Hindi
 
Dr J K Jena visit
Dr J K Jena, DDG (Fy Sci. & AS) visited ICAR DCFR Bhimtal on June 24th, 2017
 
International Yoga Day
International Yoga Day Celebration at DCFR Bhimtal & Champawat
 
World Environment Day
Mahseer Seed Ranching in Teesta River of Sikkim on World Environment Day (5th June, 2017)
 
World Environment Day
World Environment Day celebration at ICAR-DCFR, Bhimtal
 
Namami Gange Programme
Ranching of Mahseer Fingerlings in Ganga River at Laksmanjhula, Rishikesh under Namami Gange Programme
 
Swachh Bharat Pakhwada
Swachh Bharat Pakhwada at ICAR-DCFR, Bhimtal from 16 to 31.5.2017
 
Rishi Unnati Mela 2017
Salient Attractions of Krishi Unnati Mela 2017
 
Minister Visit
Shri S.S. Ahluwalia, Hon’ble Minister of State for Agriculture and Farmers Welfare visited ICAR-DCFR, Bhimtal on 2.3.2017
 
National Productivity Week
National Productivity week celebration on 12-18 February, 2017
 
Swachh Bharat Abhiyan
Swachh Bharat Abhiyan, Progress Report for the month of January, 2017
 
Agriculture Education Day
ICAR-DCFR Bhimtal and DCFR, Field Centre, Champawat organized Agriculture Education Day on 3rd December, 2016
 
World Soil Day
ICAR-DCFR Field Centre, Champawat organized World Soil Day at vill-Katharh on 05.12.2016
 
Rehabilitation programme
Rehabilitation programme at Pancheshwar by ranching fish seed of mahseer and snow trout
 
सतर्कता जागरूकता सप्ताह Report
ICAR-DCFR, observing Vigilance week during 31 October to 5 November
 
2nd October, 2016
Swachh Bharat Pakhwada at Field Centre Champawat
 
Obituary
Obituary
 
Swachh Bharat Mission
ICAR-DCFR Observes Swachhta Pakhwara under Swachh Bharat Mission- 16-31 October, 2016
 
Awareness-cum-training programme
ICAR-DCFR organized awareness-cum-training programme on trout farming in highlands on 2nd October, 2016
 
Swachhta Pakhwara Report
ICAR-DCFR Observes Swachhta Pakhwara under Swachh Bharat Mission- 2-16 Oct, 2016
 
National Interactive Meet
ICAR-DCFR, Bhimtal Convened National Interactive Meet at Jasingfaa Eco-tourism Centre, Nagaon, Assam
 
29th Foundation Day
ICAR-DCFR, Bhimtal Celebrated 29th Foundation Day
 
Interactive Meet
Interactive Meet on Mahseer in Recreational Fisheries & Eco-tourism in Northeast India
 
Awards
DCFR Nominated Farmer received the IARI-Innovative Farmers Award 2016
 
Training Programme
NFDB Sponsored Training Organized at Farm School, Toli, Champawat
 
Training Programme
ICAR-DCFR , Bhimtal organized Training Cum Demonstration Programme at Ziro Valley of Arunachal Pradesh
 
Training Programme
ICAR-DCFR and ICAR- CIFT organized Training on Fish processing and Value addition for tribal woman
 
Awareness Programme
ICAR-DCFR Field Centre, Champawat organized one day Awareness Programme on fish health management under National Surveillance programme for Aquatic Animal Diseases
 
Dissemination Programme
ICAR-DCFR Field Centre, Champawat Organized Demonstration Program on Breeding and Seed Production of Rainbow trout
 
Training Programme
ICAR-DCFR organized NFDB sponsored training & input distribution programme under NEH activity covering soil & water testing for aquaculture
 
National Workshop
National Workshop on Coldwater Endemic Fishes of North Eastern Himalaya: Avenues & Challenges on 5-6 November, 2015
 
Rainbow Trout Farming
National Consultation on “Rainbow Trout Farming: Prospects and Challenges for Strategic Development”, 20-21 September, 2015
 
Labeo Pungusia Report
Captive breeding of Labeo Pungusia, an endemic hill stream fish of North East India in PPP mode
 
Fish Farmers day
Fish Farmers day celebration at ICAR-DCFR Field Centre, Champawat
 
Workshop
ICAR-DCFR organized a workshop of Task Force on Himalayan Agriculture under the National Mission on Sustaining Himalayan Ecosystems on June 4-5, 2015
 
Training Programme
ICAR-DCFR arranged Training on Fish processing and Value addition for tribal woman
 
Intellectual Property Rights Day
Intellectual Property Rights Day celebration on 28 April, 2015
 
Training Programme
Training Programme for Fish farmers on Fish Disease Management & Control during 30 March, 2015
 
Training Programme
ICAR-DCFR Bhimtal organized NFDB sponsored Training Programme for farmers on Common Fish diseases in mid-hill fish tanks and their control during 18-22 March, 2015
 
Training Programme
ICAR-DCFR Bhimtal organized NFDB sponsored Training Programme on Prevention & Control of Diseases in Rainbow Trout during 10-14 March, 2015
 
Training Programme
ICAR-DCFR organized a Training Programme for State fishery officials at Kalimpong GTA, West Bengal and Itanagar, Arunachal Pradesh
 
Training Programme
ICAR-DCFR organized a Training Programme for farmers on "Coldwater Fish Farm Management in Mid Hills" held during 27th-31st January, 2015at Field Centre Champawat
 
Training Programme
A Training Programme on CULTURE AND BREEDING OF IMPORTANT COLDWATER FISH SPECIES
 
Training Programme
A Training Programme on MANAGEMENT PRACTICES OF JHORA FISHERIES
 
Swachh Bharat Mission
Swachh Bharat Mission
 
National Workshop
ICAR-DCFR organized National Workshop on Mahseer in India: Resources, Captive Breeding, Propagation, Policies & Issues at Guwahati
 
National Workshop
National Workshop on Mahseer in India: Resources, Captive Breeding, Propagation, Policies & Issues
 
NFDB Sponsored Training Programme
NFDB Sponsored Training Programme on Hatchery management and seed production practices of golden mahseer Organized at ICAR-DCFR
 
Swachh Bharat
DCFR follows Swachh Bharat mass movement of Honble Prime Ministers call
 
27th Foundation Day Celebration
27th Foundation Day Celebration at Directorate of Coldwater Fisheries Research on 24.9.2014
 
DCFR cage raised mahseer fingerlings
DCFR cage raised mahseer fingerlings reached Nongmahir Lake, Meghalaya for stock enhancement
 
Farmers Meet Organized on ICAR, Foundation Day
Farmers Meet Organized on ICAR, Foundation Day on 16th July 2014
 
Workshop on Intellectual Property Management
Workshop on Intellectual Property Management in Fisheries and Agriculture Sectors on July 19, 2014.
 
Fish Farmers Day
DCFR Organized Fish Farmers Day at Tribal Village Belkheda in Uttarakhand
 
Vigyan Ratn
Vigyan Ratn award was conferred to Dr. A.K. Singh, Director DCFR by the Council of Science and Technology, Government of Uttar Pradesh.
 
ICAR Sponsored Short Course
ICAR Sponsored Short Course on Application of Molecular Tools in Coldwater Fisheries Management
 
Report of Seed Ranching
Report of Seed Ranching Programme at Naukuchiatal Lake
 
Abstract book
Abstract book of National Workshop entitled "Parvatiye Chetron main Jaljeev Palan Vividhikaran
 
23-24 September, 2013
Report of Two Days National Workshop entitled "Parvatiye Chetron main Jaljeev Palan Vividhikaran" in Hindi
 
23-24 September, 2013
Two Days National Workshop entitled "Parvatiye Chetron main Jaljeev Palan Vividhikaran" in Hindi organized by DCFR, Bhimtal
 
10th July 2013
Directorate of Coldwater Fisheries Research, Bhimtal Celebrated Fish Farmers Day at village Tharu Teeshora in US Nagar, Uttarakhand
 
1 to 6 July, 2013
Upcoming Hands on Training Program on Molecular Techniques for Fish Biodiversity Analysis
 
29 March 2013
Visit of Hon’ble Minister of State for Agriculture and Food Processing Industries, Shri Tariq Anwar Ji at Directorate of Coldwater Fisheries Research, Bhimtal, Uttarakhand on 29 March 2013
Visit of Hon’ble Minister of State for Agriculture and Food Processing Industries, Shri Tariq Anwar Ji at Directorate of Coldwater Fisheries Research, Bhimtal, Uttarakhand on 29 March 2013
 
Website Inauguration
Inauguration of Official Website of DCFR, Bhimtal on 29.03.2013
Inauguration of Official Website of DCFR, Bhimtal on 29.03.2013
 
A new loach identified from Uttarakhand
A new loach identified from Uttarakhand
A new loach identified from Uttarakhand
 
Tender Notice
5th July 2018
E-Tender for Annual Rate Contract for Hiring of Vehicles at ICAR-DCFR, Bhimtal.
 
2nd June 2018
E-Tender for Annual Rate Contract for Hiring of Vehicles at ICAR-DCFR, Bhimtal.
 
Cancellation of Tender
Cancellation of Tender for Purchase of Microsoft Office Professional 2016
 
Corrigendum Notice
Corrigendum for MS Office Professional 2016 and Nitro Pro 10 and above Professional
 
14.03.2018 11 AM
Tender for painting, whitewash, cleaning etc. of office building.
 
14.03.2018 11 AM
Tender for painting, whitewash, cleaning etc. of Office Building at DCFR Field Centre, Champawat
 
13 March 2018
Tender for Purchase of Nitro Pro 10 and above Professional (10 User License)
 
Corrigendum Notice
Corrigendum for Extend of last date & time for submission of e-Tender for online subscription of the Foreign Journal.
 
06 March 2018
Tender for Purchase of Microsoft Office Professional 2016 (20 User License)
 
Recruitment
Walk in Interview
Walk in Interview will be held on 4th July 2018 for different posts on contractual basis
 
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